SUGAR-LUFF-SCHOORL METHOD 1: Scope and Field of Application This method is for the determination of glucose, reducing sugars expressed as glucose. This adaptation of the Schoorl method was designed for the determination of total The method is based on the Czech Standard ČSN 56 , Český. International Starch Institute Science Park Aarhus, Denmark ISI e Determination of Reducing Sugar, DE by Luff-Schoorl’s method.
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Make up to volume with ethanol 3. The result is expressed as the percentage content of glucose in the sample. Back to tab navigation Fetching data from CrossRef. Calculation of results Subtract the volume of silver nitrate solution 0 71 N consumed in the blank test from that consumed by the sample in solution. Remove volumes of 1, 2, 4, 5 and 10 ml merhod the urea solution 3. Compare with the blank test solution of the reagents. Previous record Next record.
Purpose and Scope This method makes it possible to determine the crude ash content of feeding-stuffs. After cooling, add water, bring the ash into suspension by means of a platinum wire, dry and ash again for two hours in the crucible with a lid.
Samples free from organic matter Weigh to the nearest mg a sample of ,uff more than 10 g and containing not more than 3 g of chlorine in the form of chlorides.
Proceedings of the Society for Analytical Chemistry. Chapter 03 Volume 6 P.
The Luff-Schoorl method for determination of reducing sugar in juices, molasses and sugar
If the magnesium content of the sample is high, precipitate the calcium oxalate a second time. This reagent keeps for a maximum period of two weeks.
If the material has been adapted instead of reproduced from the original RSC publication “Reproduced from” can be substituted with “Adapted from”. Place the Erlenmeyer immediately on an asbestos-coated wire gauze with a hole approximately 6 cm in diameter under which a flame has been lit. Using a pipette, take 25 ml of Luff-Schoorl reagent 3. Cool, neutralise exactly with the sodium hydroxide solution 3.
Redissolve the extract in 50 ml of water and bring to the boil. If the sample consists solely of mineral substances, dissolve in hydrochloric acid without ashing it first. Transfer the evaporation residue quantitatively to a ml volumetric flask using warm water, cool, bring up to volume with water, homogenise and filter if necessary. Titrate the nitric acid liberated with a solution of sodium hydroxide 3. Cool immediately in cold water methdo after approximately five minutes titrate as follows: Make the pressure equal by lowering the tube 2.
Cool and transfer the solution to a ml volumetric flask. Eliminate the ethanol as described under 5. The Luff-Schoorl method of determination of reducing sugars liff been universally adapted for the analysis of all boiling house products.
Bring to ,ethod boil, add eight to ten drops of bromo-cresol green solution 3. The formation of this precipitate causes a loss of silver nitrate solution 0 N, the volume of which must be subtracted from the volume used to calculate the HCN content.
Transfer to a ml graduated flask an aliquot part of the filtrate containing a maximum of 1 70 mg of sodium, add 10 70 ml of loading agent 3.
Procedure Weigh 20 g of the sample to the nearest 5 mg, place in 1 litre flat-bottomed flask and add 50 ml of water and 10 ml of sweet almond suspension 3. Bring slowly to the boil and boil gently for fifteen minutes. We collect your name, email address, institutional affiliation and login credentials.
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The addition of these substances largely eliminates interference from disturbing elements. Make sure that the condenser’s extension piece is immersed in the silver nitrate solution. Only special instruments or apparatus or those requiring special standards are mentioned in the description of the methods of analysis.
In the first case 17 ml of sodium thiosulphate solution 0 71 N corresponding to 44 72 mg of glucose is consumed ; in the second, 11 ml, corresponding to 27 76 schiorl of glucose.
However in certain cases for example, with iron carbonate a special method must be used. Use the Advanced search. The solution is filtered, the residue ashed and the ash thus obtained treated in accordance with Method A. If you are the author of this article you do not need to formally request permission to reproduce figures, diagrams etc. Sulphuric acid, approximately 0 71 N, coloured with methyl red.
The Luff-Schoorl method for determination of reducing sugar in juices, molasses and sugar.
Whereas all the necessary methods of analysis should be established as soon as possible ; whereas the establishment of methods for the determination of hydrocyanic acid, calcium, carbonates, crude ash, ash insoluble in HCl, chlorine from chlorides, mustard oil, lactose, potassium, sodium, sugars, theobromine and urea, and the determination of alkaloids in lupins and the estimation of the urease activity of products derived from soya are a first stage in this process.
Jump to site search. A method of reducing sugar content in molasses. One part shall be taken as it is ; the other shall be prepared as follows for chemical analysis. Calculate the result by means of a calibration curve. In the latter case it is advisible to use the method of quartering which consists of taking samples in turn from two opposite sections. Immediately add 10 ml of 0 71 N hydrochloric acid 3. Place the ash in a to ml beaker using 75 ml of hydrochloric acid 3 N 3.